Backscatter Fluorescence Yellow Barrier Filter for 67mm threads
A barrier filter’s main function is to block reflected excitation light while allowing only the emitted fluorescent light from the specimen to pass through. This filter is placed in front of the viewing system—whether it’s the human eye, a film camera, a digital camera, or a video device. The images below illustrate the effect and purpose of using the barrier filter.
1. What is a fluorescence barrier filter?
A fluorescence barrier filter is an optical filter designed to block unwanted reflected excitation light while allowing only the fluorescent emission from the specimen to pass through. This ensures a clear and accurate viewing or image capture of fluorescence.
2. Why is the filter yellow?
The yellow coloration corresponds to the wavelengths it transmits and blocks. It effectively filters out shorter wavelength (blue or ultraviolet) excitation light while allowing the longer wavelength fluorescent emission (typically green to red) to pass.
3. Where should I place the barrier filter?
The barrier filter should be positioned in front of the viewing device—such as your eye, a camera lens, or a video recorder—so that only the fluorescence signal reaches the detector or viewer.
4. Can I use this filter with any camera?
Yes. The threaded yellow barrier filter is compatible with most cameras, including film, digital, and video systems, as long as the thread size matches the camera lens.
5. What is the difference between an excitation filter and a barrier filter?
An excitation filter selects the wavelength of light used to illuminate the sample, while a barrier filter blocks that excitation light and transmits only the emitted fluorescence for observation.
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